WHERE CERTAINTY ENDS
Knowledge Gaps
A map of what is known and unknown about micro- and nanoplastics: four systems, seven questions, 46 graded findings. Microplastic presence is established in air, water, soil and human exposure; nanoplastic amounts, soil methods and removal from open systems remain the weakest answers.
IllustrationSome answers about micro- and nanoplastics are settled. Microplastics are in the air of remote mountains, in every ocean basin, in farm soils and in what people eat and breathe. Other answers are barely begun: how much nanoplastic there is, how to extract it from soil, and whether any method removes particles from open air, water or soil.
This map grades 46 checkable findings across four systems (air, water, soil, human body) and seven questions. Microplastics (MP, 1 µm–5 mm) and nanoplastics (NP, below 1 µm) are graded separately wherever their evidence differs, so a weak nano result does not pull down an established micro one.
One number shows how thin the nano layer is. About 27 million metric tons (27 Mt) of nanoplastic in the North Atlantic mixed layer is an extrapolation from nine open-ocean stations sampled on one cruise in November 2020 (level C)1.
27 Mt
nanoplastic mass estimate, North Atlantic mixed layer
PET, PS and PVC below 1 µm; mixed-layer sum for stations 1–9 (12 stations sampled, three on the European shelf); RV Pelagia cruise 64PE480, November 2020; TD-PTR-MS; spatial extrapolation1
one transect; polyethylene and polypropylene were not detected
CLimited283
locations behind the global dataset of airborne microplastics
2,782 concentration and deposition measurements from 76 studies; coordinates rounded to four decimals (one Wenzhou site duplicated by floating-point noise in the raw file)2,3
the compiled data describe sizes from several µm to several mm
PProvenance0
measurement locations on the African mainland in that dataset
our count in the published dataset, 3 November 2026; two Canary Islands sites are coded as Atlantic Ocean3
our calculation; a sampling gap, not evidence of clean air
PProvenance1.4%
overall recovery of a particle-preserving nanoplastic extraction from soil
Forsyth et al. 2026; palladium-doped nanoplastic spikes; extraction, density separation, filtration below 1 µm and ultrafiltration combined4
single laboratory study; the steps alone recovered 38–92%
CLimitedHow to read the map
Each row is one finding, stated so that it can be checked: a system, a question, the size class and the claim. Occurrence asks whether particles are present and, in a separate row where it matters, how much; sources, where they come from; transport, how they move (for the human body, uptake and distribution); effects, what they do; methods, whether they can be measured reliably and comparably; removal, whether a tested method takes them out; regulation, which rules exist.
The level grades the evidence for that finding, not whether the news is good or bad. E means not established in the evidence we reviewed, with the search recorded below. Rule rows carry P: they are legal facts checked against the official text, not graded science. The rubric is in Evidence Levels.
The knowledge map: four systems, seven questions
| System | Question | Level | Why this level | Sources |
|---|---|---|---|---|
| air | occurrence · MP: present in outdoor air, from cities to remote and polar sites | AConsensus | Independent studies in Europe, North America and the Arctic; European academies review lists air as contaminated | 5,6,7,2,8 |
| air | occurrence · MP: global concentrations quantified | CLimited | 283 locations, 35 southern, none on the African mainland; values span orders of magnitude | 2,3 |
| air | occurrence · NP: present in remote snow and ice | CLimited | Alpine snow and polar ice, measured by one research group with one method (TD-PTR-MS) | 9,10,11 |
| air | sources · MP: land versus ocean emission shares | DDisputed | Emission inventories disagree; models exceed measured concentrations 100- to 10,000-fold | 2,12,13 |
| air | transport · MP: long-range transport to remote regions | AConsensus | Remote deposition documented independently on several continents; European academies review concurs | 5,6,7,8 |
| air | transport · MP+NP: fluxes and residence times | CLimited | Few flux measurements; nanoplastics tracked by one method in snow and ice | 14,10,11 |
| air | effects · MP+NP: direct radiative forcing | CLimited | Three model studies, 2021–2026; sign and size depend on color, size and concentration assumptions | 15,16,17,18 |
| air | methods · MP+NP: comparable ambient measurement | CLimited | No air standard found; compiled studies differ in size windows; nanoplastics only by research instruments | 2,9,19 |
| air | removal · MP+NP: removal from outdoor air at scale | EUnknown | Not established in evidence reviewed; indoor HEPA filters retain only particles in the air passing through | 20,21 |
| air | regulation · rules in force | PProvenance | Euro 7 frames tire-abrasion limits at the source; no ambient-air plastic limit in the EU acts checked | 22 |
| water | occurrence · MP: present in every ocean basin, lakes and rivers | AConsensus | Many independent surveys; European academies (2019) and Thompson et al. (2024) reviews concur | 23,24,25,8,21 |
| water | occurrence · NP: present in seawater and fresh water | BWell supported | Independent groups detected nanoplastics in the North Atlantic (2017, 2025) and Chinese surface water and groundwater | 26,1,27 |
| water | occurrence · NP: ocean mass inventory | CLimited | 27 Mt extrapolated from nine open-ocean stations on one 2020 cruise; PE and PP undetected | 1 |
| water | sources · MP: land and river inputs to the ocean | CLimited | Inputs come from models, not measured budgets | 28,29 |
| water | sources · NP: origin of open-ocean nanoplastics | EUnknown | Authors of the only basin-scale profile call the origin open; no open-ocean source apportionment found | 1,30 |
| water | transport · MP+NP: sinking, sea-spray and deep fluxes | CLimited | River pathways modeled; sea-spray export from laboratory experiments; deep fluxes sparsely measured | 29,31,32,33,14 |
| water | effects · MP: effects on aquatic organisms in laboratory tests | BWell supported | Many tests; a quality review of 105 retains food dilution and physical damage as mechanisms | 34,21 |
| water | effects · MP: effects at field-realistic exposure | CLimited | None of 105 aquatic studies met all quality criteria; exposure relevance and size mismatch are main weaknesses | 34 |
| water | effects · NP: dose-effect relationships | CLimited | European academies (2019): most nanoplastic designs could not establish a dose-effect relationship | 8 |
| water | methods · MP ≥ 20 µm: standardized measurement | BWell supported | ISO 16094-2 and the EU method adopted; interlaboratory exercises found spread, largest for small particles | 35,36,37,38,39 |
| water | methods · NP: validated mass measurement | CLimited | Research methods (py-GC/MS, TD-PTR-MS) used by few groups; no standard or interlaboratory study found | 1,40,27 |
| water | removal · MP: retention by wastewater treatment | AConsensus | WHO (2019) reports removal above 90%; independent plant studies agree | 41,40 |
| water | removal · NP: retention by treatment | CLimited | Three Australian plants cut nano mass 91–96%; pilot filtration retained metal-doped model particles | 40,42 |
| water | removal · MP+NP: removal from open water | EUnknown | Not established in evidence reviewed; ocean cleanup targets floating macroplastic | 43,21 |
| water | regulation · rules in force | PProvenance | EU method 20 µm–5 mm; California Phase I reports from 50 µm; no limit value; nanoplastics outside both | 36,44,45 |
| soil | occurrence · MP: present in soils, including farmland | AConsensus | European academies review and FAO assessment agree with many soil studies | 8,46,47 |
| soil | occurrence · MP: comparable concentrations across soils | CLimited | Review finds soil methods differ, so concentrations are hard to compare | 48 |
| soil | occurrence · NP: present in field soil | CLimited | Few field data; polystyrene nanoplastics measured at one compost-amended French site by matrix-matched py-GC/MS (2.57 µg/g in the 200–1,000 nm topsoil fraction, about 28 times a reference plot); a second French field gives only an isotope estimate of the mobile fraction (about 0.25 µg/kg to 55 cm) | 49,50 |
| soil | occurrence · NP: representative multi-polymer mass across farm soils | EUnknown | Not established in evidence reviewed; the one field quantification we read covers one polymer at one site | 49,50 |
| soil | sources · MP: sludge, mulch film and compost | BWell supported | FAO covers mulch film and compost; sludge rests on separate studies; amounts are estimates | 46,51,47 |
| soil | transport · NP: uptake into plants | CLimited | Laboratory beads enter crop roots; one field study found polystyrene mostly in roots, shoots below LOQ | 52,53,49 |
| soil | effects · MP+NP: soil structure, microbes and crops | CLimited | Laboratory and outdoor-mesocosm studies; direction varies by polymer, shape and dose | 54,47,55,53 |
| soil | methods · MP: standardized, interlaboratory-tested method | CLimited | No published standard or interlaboratory results found; review finds methods incomparable | 48 |
| soil | methods · NP: validated extraction and quantification | CLimited | Polystyrene-specific py-GC/MS method tested with spikes; particle-preserving extraction recovered 1.4% | 56,4 |
| soil | removal · MP+NP: removal from field soil | EUnknown | Not established in evidence reviewed; biochar reduced lettuce uptake in one greenhouse study | 57 |
| soil | regulation · rules in force | PProvenance | EU Soil Monitoring Law allows plastic monitoring, sets no plastic method or limit; REACH restricts products | 58,59 |
| human | occurrence · MP: present in human tissues | BWell supported | Independent groups using different methods report particles in blood, brain and arterial plaque | 60,61,62,63 |
| human | occurrence · MP+NP: tissue mass concentrations | DDisputed | Brain and blood values disputed over lipid interference and contamination; authors defend their data | 61,64,65,66,60 |
| human | sources · MP: exposure through food, water and air | AConsensus | Two WHO assessments and an EFSA panel statement concur | 41,67,68 |
| human | sources · MP: weekly intake mass | DDisputed | Model estimates span about 4 µg to 5 g per week, with different size windows and conversions | 69,70,67 |
| human | transport · NP: uptake, distribution and clearance | CLimited | Shown with model beads in animals; WHO (2022) found human data insufficient | 67,71,72 |
| human | effects · MP+NP: association with disease | CLimited | One cohort (257 followed): plaque plastic associated with heart attack, stroke, death; causation unshown | 63,73 |
| human | methods · MP+NP: blood quantification by py-GC/MS | DDisputed | Rauert: unsuitable for PE and PVC in blood; Nardella: detected in all 102, quantified in 20% | 66,74,75 |
| human | removal · MP: lowering circulating particles clinically | CLimited | One uncontrolled study, 114 patients; tubing shed particles; benefit and nano removal not established | 76,77 |
| human | regulation · rules in force | PProvenance | No health-based guidance value adopted by WHO or the EU found | 41,67 |
| human | regulation · MP+NP: evidence basis for a guidance value | BWell supported | WHO 2022: insufficient for full assessment; WHO 2019: drinking water low concern, nano data insufficient | 67,41,8 |
Knowledge map: evidence level of each finding, by system and question
Data table
| label | value | unit | system | question | size_class |
|---|---|---|---|---|---|
| air · occurrence · MP present | A | air | occurrence | MP | |
| air · occurrence · MP quantified | C | air | occurrence | MP | |
| air · occurrence · NP present | C | air | occurrence | NP | |
| air · sources · MP shares | D | air | sources | MP | |
| air · transport · MP long-range | A | air | transport | MP | |
| air · transport · fluxes | C | air | transport | MP+NP | |
| air · effects · radiative forcing | C | air | effects | MP+NP | |
| air · methods · comparability | C | air | methods | MP+NP | |
| air · removal · outdoor | E | air | removal | MP+NP | |
| air · regulation · rules | P | air | regulation | MP+NP | |
| water · occurrence · MP present | A | water | occurrence | MP | |
| water · occurrence · NP present | B | water | occurrence | NP | |
| water · occurrence · NP ocean mass | C | water | occurrence | NP | |
| water · sources · MP inputs | C | water | sources | MP | |
| water · sources · NP origin | E | water | sources | NP | |
| water · transport · vertical and air-sea fluxes | C | water | transport | MP+NP | |
| water · effects · MP laboratory | B | water | effects | MP | |
| water · effects · MP field-realistic | C | water | effects | MP | |
| water · effects · NP dose-effect | C | water | effects | NP | |
| water · methods · MP standard | B | water | methods | MP | |
| water · methods · NP mass | C | water | methods | NP | |
| water · removal · MP wastewater | A | water | removal | MP | |
| water · removal · NP treatment | C | water | removal | NP | |
| water · removal · open water | E | water | removal | MP+NP | |
| water · regulation · rules | P | water | regulation | MP+NP | |
| soil · occurrence · MP present | A | soil | occurrence | MP | |
| soil · occurrence · MP comparable | C | soil | occurrence | MP | |
| soil · occurrence · NP present (field) | C | soil | occurrence | NP | |
| soil · occurrence · NP representative mass | E | soil | occurrence | NP | |
| soil · sources · MP inputs | B | soil | sources | MP | |
| soil · transport · NP plant uptake | C | soil | transport | NP | |
| soil · effects · structure, microbes, crops | C | soil | effects | MP+NP | |
| soil · methods · MP standard | C | soil | methods | MP | |
| soil · methods · NP extraction | C | soil | methods | NP | |
| soil · removal · field soil | E | soil | removal | MP+NP | |
| soil · regulation · rules | P | soil | regulation | MP+NP | |
| human · occurrence · MP present | B | human | occurrence | MP | |
| human · occurrence · tissue mass | D | human | occurrence | MP+NP | |
| human · sources · exposure routes | A | human | sources | MP | |
| human · sources · intake mass | D | human | sources | MP | |
| human · transport · NP uptake | C | human | transport | NP | |
| human · effects · disease association | C | human | effects | MP+NP | |
| human · methods · blood py-GC/MS | D | human | methods | MP+NP | |
| human · removal · plasma exchange | C | human | removal | MP | |
| human · regulation · rules | P | human | regulation | MP+NP | |
| human · regulation · evidence basis | B | human | regulation | MP+NP |
Two patterns run across the rows. Microplastic presence reaches A in every environmental system, while every nanoplastic finding sits at B or below.
Removal splits the same way: wastewater treatment retains most microplastics (A) and, in three plants, most nanoplastic mass (C), but in the evidence we reviewed no method has been shown to remove dispersed particles from outdoor air, open water or field soil (E). That matches the 0 / 4 finding in Solutions by System. The human removal row is a different question, a clinical one, and stays at C76.
The five biggest gaps
Ocean nanoplastic mass rests on one transect
Ten Hietbrink and colleagues found PET, polystyrene (PS) and polyvinyl chloride (PVC) nanoplastics at about 1.5–32.0 mg/m³ at all 12 stations, surface to bottom (level C)1. The 27 Mt estimate sums the mixed layer at the nine open-ocean stations, not the three on the shelf. Polyethylene (PE) and polypropylene (PP), about half of global plastic production, were not detected: the authors' "missing PE and PP nanoplastic paradox"1. We found no second basin-scale profile to test it.

The air map has blank continents and no nano layer
Evangelou and colleagues compiled 2,782 measurements of airborne microplastics from 76 studies at 283 locations (level P)2. In the published dataset we count no location on the African mainland, one in South America and 35 in the Southern Hemisphere (our calculation from3). The authors describe reported sizes from several micrometers to several millimeters and ask for measurements extended to smaller microplastics and nanoplastics2.
Where airborne microplastics have been measured
Data table
| label | value | unit | measurements |
|---|---|---|---|
| South and Southeast Asia (SAS) | 101 | locations | 501 |
| Europe (EUR) | 56 | locations | 652 |
| East Asia (EAS) | 46 | locations | 406 |
| North America (NAM) | 22 | locations | 751 |
| Antarctica and Southern Ocean (ANT) | 20 | locations | 42 |
| West and Central Asia (WAS) | 15 | locations | 324 |
| Australia and Pacific (AUS) | 13 | locations | 42 |
| Atlantic Ocean (ATL) | 9 | locations | 24 |
| South America (SAM) | 1 | locations | 40 |
| African mainland | 0 | locations | 0 |
How much plastic is inside a human body
Independent groups using different methods find particles in human tissue (level B). In April 2026 Li and colleagues reported micro- and nanoplastics in 99.4% of 156 brain samples from 113 patients with brain tumors and in all 35 samples from five healthy post-mortem donors, combining infrared imaging (LDIR, O-PTIR) with py-GC/MS and testing operating-room contamination62. A correction fixed figure rounding only78. Healthy tissue came from five people, and the link to tumor growth is a correlation.
How much plastic is there is disputed (level D). Nihart and colleagues reported brain concentrations by py-GC/MS, supported by infrared spectroscopy and electron microscopy with elemental analysis61. Critics argue that brain lipids can produce false polyethylene signals64. The authors' reply defends the data: per a 2026 summary, the lipid comparison mixes dry- and wet-weight percentages, polymers without known lipid interference rose too, and blanks held no detectable polymer; we could read only the reply's preview65,79.
In blood, Rauert and colleagues judged py-GC/MS currently unsuitable for PE and PVC, with extraction recoveries of 7–109%66. Nardella and colleagues detected plastic in all 102 blood samples but quantified it in only 20%74. Detection is not a validated dose. See Human Body.
Toxicity tests and exposure data describe different particles
The World Health Organization (WHO) found that most occurrence data for water, food and beverages concern particles larger than 10 µm, while most toxicity studies used uniform particles below 10 µm (level A)67.
A quality review of 105 aquatic effect studies found the same mismatch between tested and environmental sizes; no study met all 20 criteria, and environmental relevance of exposure was among the most urgent weaknesses34. WHO adds that hazard data after inhalation or ingestion were limited to polystyrene beads67.
Soil nanoplastic methods recover little
Field measurements now exist, but they are narrow. In a French floodplain amended with compost for decades, Ryzhenko and colleagues measured polystyrene nanoplastics by matrix-matched py-GC/MS: 2.57 µg/g in the 200–1,000 nm fraction of the topsoil, about 28 times a reference plot, 123 µg/g in grass roots and 32 µg/g in shoots, the shoot value below the quantification limit (level C)49. That is one polymer at one site.
At a second compost-amended French field, an isotope estimate put the mobile fraction in soil water at about 0.25 µg/kg down to 55 cm, not the total in soil (level C)50.
Methods are the bottleneck: a polystyrene-specific py-GC/MS method has been tested with spiked soils and sediments56, while a particle-preserving extraction recovered 1.4% overall, too little for quantitative analysis4.
Why the gaps persist
The first reason is size. Methods that identify polymers particle by particle rarely go below about 1 µm; below that, laboratories mostly use thermal methods that report polymer mass without particle size or count19. Method detail is in How We Measure It.
The second is comparability. When 12 laboratories analyzed the same sample bottles, their results differed widely, most for small particles (here, below 1 mm)37. A drinking-water interlaboratory study also found poorer recovery of small particles39.
The third is contamination. The North Atlantic team ran laboratory blanks and notes that whether clean rooms eliminate plastic contamination at the nanoscale is uncertain1.
The fourth is access. Human samples come from surgery, autopsy or blood draws, and WHO found too little information to assess how particles are taken up, retained and cleared67.
The size windows of current methods show the problem at a glance.
Which particle sizes current methods and rules cover
Data table
| label | value | unit | range_from | range_to | source |
|---|---|---|---|---|---|
| Nanoplastics (site convention) | 1 | µm | 0.001 | 1 | S-017 |
| North Atlantic nanoplastic study: fraction passing a 1.0 µm filter (lower size bound not stated) | 1 | µm | 1 | S-067 | |
| Soil nanoplastic extraction (Forsyth et al.): filtration below 1 µm | 1 | µm | 1 | S-288 | |
| California drinking-water monitoring, Phase II (planned, treated water): down to 5 µm | 5 | µm | 5 | 5000 | S-194 |
| EU drinking-water method: particles 20 µm–5 mm | 20 | µm | 20 | 5000 | S-132 |
| California drinking-water monitoring, Phase I (source water): reporting from 50 µm or the method minimum (20 µm by Raman) | 50 | µm | 20 | 5000 | S-194 |
| California definition of microplastics in drinking water | 5000 | µm | 0.001 | 5000 | S-194 |
| Microplastics: upper bound | 5000 | µm | S-017 |
The field is also young at the nano scale. In 2019 the European academies found no studies of atmospheric nanoplastic pollution and no analytical method for nanoplastics in soil8. Nanoplastics were then reported in Alpine snow in 2020 and 2021 and in polar ice in 20229,10,11. In soil, field measurement of nanoplastic mass is still rare; we read one quantification, from 202649, and one isotope estimate of the mobile fraction, from 202450.
What would close them
Five steps would move the most findings.
- Nanoplastic methods validated with orthogonal techniques and reported with confidence levels75, including soil extractions that recover enough to quantify4.
- Effect studies with environmentally realistic particles and exposures, and reference particles that resemble environmental ones, as WHO and the quality review recommend67,34.
- Size-resolved air monitoring that extends to smaller microplastics and nanoplastics2, with sites outside Europe, North America and Asia (our reading of3).
- Repeat ocean transects that test the North Atlantic estimate and look for the missing PE and PP1.
- Human studies whose exposure is measured by validated methods, so that associations such as the plaque cohort can be tested63,75.
Thompson and colleagues add that clear evidence on whether proposed solutions work is now needed21. The Lancet Countdown on health and plastics has begun an indicator-based monitoring system that could track some of these gaps over time80.
Search record for the E findings
We searched PubMed on 3 November 2026 (publication years 2015–2026, English titles and abstracts), screened every title and read the abstracts of candidates that looked like field-scale evidence. Exact search strings are kept in the claims record of this page.
- Removal from outdoor air: 129 records; none tested removal from outdoor air at scale. Excluded: reviews, indoor and wastewater studies, an urban-forest interception perspective.
- Origin of open-ocean nanoplastics: 12 records; none apportioned open-ocean sources. One coastal Mediterranean study traced low-molecular-weight PE and silicone debris (retained on a 0.45 µm filter) to sources compatible with wastewater and consumer products; it does not separate nano from micro particles.
- Removal from open water: 33 records; none showed removal from a lake, river or ocean. Excluded: laboratory adsorbents and one lake pilot that retained microplastics from pumped water only.
- Representative nanoplastic mass in farm soils: 51 records; one field study of one polymer49. Excluded: pot experiments, reviews and a 120-field survey that reports total plastic mass below 5 mm without a nano fraction.
- Removal from field soil: 54 records; none showed removal at field scale. Excluded: pot, laboratory and review studies.
A missed study would change a grade; please send it.
How this map is maintained
Method
We regrade a finding when a peer-reviewed study, interlaboratory comparison, standard or agency assessment changes what is known; dated change notes are planned, and no regrades have been recorded yet. A finding moves up only with new independent data, not with a press release. You can propose a regrade, with a source, through Corrections & Contact. Last full review: 3 November 2026.
Gallery
IllustrationSources
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